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The Aaron Klug Centre for Imaging and Analysis

The Protein Purification Lab is run by Dr Brandon Weber, various instruments are located in the lab and can be booked after consultation with Brandon.

Protein Expression and Purification Laboratory

  • Spectrophotometer and multiskan plate reader

    Thermo Scientific™ Multiskan™ GO Microplate Spectrophotometer allows you to perform photometric research applications such as DNA, RNA and protein analysis. The Multiskan GO plate reader features a broad wavelength range (including UV area), path length correction and a fast reading speed. In addition you can run quick measurements directly from the instrument.

    Helios Spectrophotometers’ superior optical system provides stability, precision and linearity. All units feature angled casing, sealed keypad to protect from spills, optional printer, plug-and-play self-aligning lamps, large LCD, VGA graphics, full-range scan displayed on-screen with peak pick-and-track facilities, choice of total control or PC-driven software, built-in methods storage, cell volume handling to 5 µL and 100% sealed optics to protect from dust and vapors.

    Spectrophotometer and multiskan plate reader

  • Akta Explorer Chromatography system

    ÄKTAexplorer has been widely used in the industry for fast and robust method and process development for over a decade. Its versatility and reliable operation made it an excellent choice for laboratories involved in developing purification processes

    Akta Explorer Chromatography system

  • Akta Prime Chromatography system

    ÄKTA prime plus is a flexible system that can be used for more general purification tasks such as buffer exchange, gel filtration, ion exchange, HIC, and affinity chromatography. The system has preprogrammed methods for typical applications such as purification of affinity tagged proteins, MAbs, and sample clean-up.

    Akta Prime Chromatography system

  • Gilson Dual Wavelength Liquid Chromatograph

    Gilson's 15X Series of UV/VIS (UVVis / UV-Vis) Detectors deliver versatile, rugged detection for HPLC. Features:

        Wavelength range 190-700 nm
        Wavelength accuracy ± 1 nm
        Wavelength reproducibility ± 0.2 nm
        Sensitivity range 0.001 - 2.0 AUFS
        Single wavelength mode
        Dual wavelength mode (155)
        Scan wavelength mode
        Deuterium source lamp
        Standard analytical flow cell volume 10 ul
        Autozero, mark, time constant
        Signal output 1-1000mV

    Gilson Dual Wavelength Liquid Chromatograph

  • Gilson SEC HPLC

    Analytical chromatography is nornally done with small amounts of material to establish the presence or measuring the relative proportions of chemical constituents in a mixture. Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers.

  • Gilson SEC Fridge HPLC

    Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. Some proteins are temperature sensitive, so in order to maintain protein stability, we have a chromatography system in a fridge which maintains the samples at a constant low temperature.

    Gilson SEC Fridge HPLC