Search

The Aaron Klug Centre for Imaging and Analysis

Home > Instruments and services > Light microscopes

Light and Fluorescent Microscopes

  • Wild M400 Photomacroscope

    Wild microscope

    Universal-macroscope for quality inspection, measuring and documentation.

    The M400 features a 5-fold zoom objective which enables a large field of view and a large working distance at the same time. Ideal for:

    • ambitious microphotography
    • precise measurement
    • inspection in polarized light

    Features

    • vertical optical path
    • phototube with 1-fold C-mount 1x (within range of delivery)
    • high resolution at large working distance and large field of view
    • precise rough/fine drive for fast and fine focus adjustment
    • built-in iris aperture, aperture diaphragm
    • fibre glass-coaxial illumination module
    • ancillary lense 2,0-fold
    • transmitting light stand
    • xy stage/ measuring stage
    • software for image measuring

    Links

  • Nikon Diaphot Inverted Fluorescent Microscope

    As opposed to the standard configuration of an upright microscope in which the ocular and objective are located above the specimen stage, the lenses of an inverted microscope are located below the viewing stage, with the light source illuminating the subject from above. The specimen is located face down above the objective with the line of sight directed upward through the objective to the object. The Nikon Diaphot allows for the inclusion of a digital camera for viewing on a CCTV monitor. We have three fluorescent filters to excite the following wavelengths:

    Excitation wavelength Colour of fluorescence Excitation method Useful Fluorochromes
    330-380 nm Blue Ultraviolet Hoescht, DAPI
    450-490 nm Green Blue Light FITC
    510-560 nm Red Green Light TRITC

    Applications

    nikon

    Inverted microscopes are among the most ubiquitous analytical instruments available and are most commonly used for examining thick samples and specimens that typically settle on the bottom of observation dishes. Phase contrast, fluorescence, multidimensional imaging, Nomarski DIC and time-lapse imaging are just some of the viewing techniques used with inverted microscopes in the biological sciences for the study of living cells, tissue cultures and parasitic organisms. Industrial applications for materials science and engineering laboratories include the three-dimensional analysis of metals and semiconductor materials, checks for surface defects, grain size and incursions in QA/QC, and the analysis of surface microstructures in a variety of sample types in metallurgy and metallography.

    Fluorescence imaging allows molecules beyond the resolution limit of the light microscope to be visualized. Fluorescence microscopy is a key technique in clinical diagnostic (for example, immunology, pathology, microbiology, cytogenetics) and research environments.

  • Leitz Diaplan with Hoffmann modulation contrast optics

    This microscope is equipped with brightfield, darkfield, phase contrast and an incident light fluorescence illuminator, as well as a temperature control system (5-120°C).

  • Zeiss Axiocam Digital Camera

    The Zeiss Axiocam Digital Camera and Axiovision software enable people to acquire high quality digital images from our various light microscopes including the Leitz Diaplan with Hoffmann modulation contrast optics and the Wild M400 Photomacroscope.

  • Various other light microscopes

    Various other light microscopes